Kris Carr explains why pH balance in the body is essential with high alkaline foods and an acid alkaline balance through a ph balanced diet.

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. Objective. To standardize a procedure for the enzymatic assay of alkaline phosphatase, diethanolamine assay. Scope. 2. 1 This procedure applies to all products that have a specification for alkaline phosphatase utilizing the diethanolamine assay system. This enzyme assay is not to be used to assay alkaline phosphatase in which the specific activity is cited only in glycine units. Definitions. 3. 1 Purified Water - Water from a deionizing system, resistivity ~1. M. Discussionp- Nitrophenyl Phosphate + H2.

O    Alkaline Phosphatase   > p- Nitrophenol + Pi. Responsibilities. It is the responsibility of all trained Analytical Services personnel to follow this protocol as written.

Alkaline Phosphatase, Serum CPT Test code: 84075 Specimen: Serum (preferred) or plasma Volume: 2 mL Minimum Volume: 0.5 mL Container. Advantages and Features Features Rapid and irreversible heat inactivation eliminates unwanted activity; Improved storage stability versus native enzyme.

Safety. Refer to Safety Data Sheets (SDS) for hazards and appropriate handling precautions. Procedure. 7. 1 CONDITIONS: T = 3.

Adjust the p. H of the diethanolamine to 9. Utilisation De Microsoft Visio Pdf Manual. C with 5 M HCl. Dilute p. H adjusted solution to 1.

L Diethanolamine final concentration with purified water and add 0. L/L of 1. M magnesium chloride solution, such as Sigma product number M1. Prepare fresh and protect from light. M p- Nitrophenyl Phosphate Solution (PNPP)Prepare a 2. L solution in purified water using Phosphatase Substrate, such as Sigma product number P4.

Prepare fresh and protect from light. Alkaline Phosphatase Enzyme Solution (Enzyme)7. Immediately before use, prepare a solution containing approximately 0. L of alkaline phosphatase in cold Buffer. For liquid samples, prepare a concentrated (2. L or greater) stock solution in cold buffer immediately before use.

Perform subsequent dilutions to achieve final solution containing approximately 0. L in cold Buffer.

TEST METHOD: 7. 4. Pipette (in milliliters) the following reagents in the following sequence into the appropriate cuvette: Test 1.

Test 2. Test 3. Blank. Buffer (Reagent 7. PNPP (Reagent 7. 3. Mix by inversion and equilibrate to 3.

C. Monitor the A4. Then add: Test 1.

Test 2. Test 3. Blank. Enzyme (Reagent 7. Immediately mix by inversion and record the increase in A4. Obtain the . References. Walter, K. Moessner, M.

Pfleiderer, Hoppe- Sayeler’s Z. April 1. 98. 0. 9. Approval. Review, approvals and signatures for this document will be generated electronically using the EDMS. Print a “For Use” copy if hardcopy with signature verification is required.

Alkaline Phosphatase. ALKALINE PHOSPHATASEAlkaline Phosphatases are a group of enzymes found primarily the liver (isoenzyme ALP- 1) and bone (isoenzyme ALP- 2). There are also small amounts produced by cells lining the intestines (isoenzyme ALP- 3), the placenta, and the kidney (in the proximal convoluted tubules).

What is measured in the blood is the total amount of alkaline phosphatases released from these tissues into the blood. As the name implies, this enzyme works best at an alkaline p. H (a p. H of 1. 0), and thus the enzyme itself is inactive in the blood. Alkaline phosphatases act by splitting off phosphorus (an acidic mineral) creating an alkaline p. H. The primary importance of measuring alkaline phosphatase is to check the possibility of bone disease or liver disease. Since the mucosal cells that line the bile system of the liver are the source of alkaline phosphatase, the free flow of bile through the liver and down into the biliary tract and gallbladder are responsible for maintaining the proper level of this enzyme in the blood. When the liver, bile ducts or gallbladder system are not functioning properly or are blocked, this enzyme is not excreted through the bile and alkaline phosphatase is released into the blood stream.

Thus the serum alkaline phosphatase is a measure of the integrity of the hepatobiliary system and the flow of bile into the small intestine. In addition to liver, bile duct, or gallbladder dysfunction, an elevated serum alkaline phosphatase can be due to rapid growth of bone since it is produced by bone- forming cells called osteoblasts. One would expect that growing children have higher levels than full- grown adults. The relationship of alkalinity to bone development warrants further discussion because it plays a major role in the prevention and reversal of osteoporosis. Just as calcium builds up around faucets, so is calcium laid down into bone.

The reason the calcium deposits on your faucet is because the water is alkaline and calcium comes out of solution and crystallizes in an alkaline environment. The reverse is also true, “Lime - Away”, vinegar, or any other acidic solution dissolve the calcium deposits because they are acidic. It makes sense that osteoblasts by creating a local environment of alkalinity via alkaline phosphatase helps build bone.

It also implies that in order to slow bone loss, one can not be in an acidic state. Studies have shown that giving bicarbonate of potassium is just as effective as calcium in correcting osteoporosis! One would expect then that in an acidic state, the body will compensate for this by increasing the bone alkaline phosphatase levels.

Associated organs/glands include adrenals, uterus, prostate, and intestine. The consequences of impaired bile flow are pervasive since bile is critical to your body’s ability to process fats. As a result, fats remain undigested in the digestive tract and can cause bloating, cramps, light colored stools, gaseousness, etc. Many patients report pressure or pain in the right upper area of their abdomen where the liver and gallbladder are located. You may have discomfort in the right shoulder or between your shoulder blades anywhere from your mid- back to the base of your neck.

Many people say they “carry my stress in the upper back and neck.” This may due to gallbladder dysfunction. Unfortunately, a normal alkaline phosphatase does not exclude hepatobiliary dysfunction. In many cases, even the ultrasound shows no gallstones, etc.

Rather the problem is that the bile does not flow freely throughout the system, which may result in insufficient bile action. The consequences of impaired bile function involve the endocrine system in a major way because all of the steroid hormones are metabolized in part by the liver. These include the sex hormones (androgens and estrogens). As a result the menstrual cycle, sexual functions and sex characteristics can be affected. The optimal range for alkaline phosphatase depends on your age.

A growing adolescent will have a much higher alkaline phosphatase than a full grown adult because his/her osteoblasts are laying down bone very rapidly. For an adult, 5. 0- 7. An increased serum Alkaline Phosphatase may be due to: Congestion or obstruction of the biliary tract, which may occur within the liver, the ducts leading from the liver to the gallbladder, or the duct leading from the gallbladder through the pancreas that empty into the duodenum (small intestine). Any of these organs (liver, gallbladder, pancreas, or duodenum) may be involved. Liver congestion/cholestasis Oral contraceptives.

Obstructive pancreatitis. Hepatitis/Mononucleosis/CMVCongestive heart failure. Parasites. Malignancy involving liver*See the lab section on liver enzyme tests for more about causes of liver dysfunction. Osteoblastic/Bone Conditions Paget’s Disease. Herpes Zoster (Shingles)Hyperthyroidism. Over- activity of the Parathyroid glands (Primary Hyperparathyroidism, Secondary Hyperparathyroidism from kidney disease, osteomalacia, malabsorption)Rickets – Vitamin D deficiency. Healing fractures, rapid bone growth Rapid bone growth such as after a fracture, bone cancers like osteogenic sarcoma, Osteomalacia, and Paget’s Disease.

Osteoporosis treatment. Adrenal cortical hyperfunction. Non- Bone/Non- Liver Conditions. As a normal part of late pregnancy since the placenta produces alkaline phosphatase (placenta – ~2x normal)Amyloidosis. Granulation tissue.

Gastrointestinal inflammation (Inflammatory Bowel Disease: Ulcerative colitis, Crohn’s; ulcers)Systemic infections (sepsis)Sarcoidosis. Rheumatoid arthritis.

Certain cancers such as Hodgkin’s Lymphoma, gynecologic malignancies. Acute tissue damage in the heart or lungs (myocardial or pulmonary infarctions). An elevated alkaline phosphatase almost always requires other tests to determine the origin of the condition. For example, liver enzyme tests to check the integrity of the liver, x- rays or other bone images if a bone abnormality is evident.

Although not used often, the isoenzyme profile of alkaline phosphatases can be determined to see if the elevation of alkaline phosphatase came primarily from liver (ALP- 1), bone (ALP- 2), or elsewhere. Most often, however there is a modest elevation from ideal but the actual value is within the laboratories reference range and the origin is inferred from the symptoms, exam, or existing lab results. A decreased serum alkaline phosphatase may be due to: Zinc deficiency. Hypothyroidism. Vitamin C deficiency/Scurvy.

Folic acid deficiency. Excess Vitamin D intake. Low phosphorus levels (hypophosphatasia)Celiac disease. Malnutrition with low protein assimilation (including low stomach acid production/hypochlorhydria). Insufficient Parathyroid gland function.

Pernicious anemia. Vitamin B6 insufficiency.